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Generation of Expressed Sequence Tags (ESTs) for Gene Discovery and Marker Development in Cultivated Peanut

^a USDA-ARS, Crop Protection and Management Research Unit, Tifton, GA 31793
^b Univ. of Georgia Coastal Plain Experiment Station, Tifton, GA 31793
^c USDA-ARS, U.S. Horticultural Research Lab., Ft. Pierce, FL 34945
^d Center for Plant Biotechnology Research, Tuskegee Univ., Tuskegee, AL 36088
^e USDA-ARS, Crop Genetics and Breeding Research Unit, Tifton, GA 31793

View larger version (14K): [in this window] [in a new window]   Fig. 1. Distribution of ESTs from leaf and immature cDNA libraries among functional categories. MIPS functional categories: 1-Metabolism; 2-Energy; 3-Cell growth, division and DNA synthesis; 4-Transcription; 5-Protein synthesis; 6-Protein destination; 7-Transport facilitation; 8-Cellular transport and transport mechanisms; 9-Cellular biogenesis; 10-Cellular communication/signal transduction mechanism; 11-Cell rescue, defense and virulence; 12-Ionic homeostasis; 13-Cellular organization; 14-Development; 15-Unclassified protein.

View larger version (78K): [in this window] [in a new window]   Fig. 2. Quantitative expression analysis of genes with putative function of disease resistance and drought tolerance by reverse transcription PCR (RT-PCR). Total RNA was extracted from peanut C34-24 leaf samples of healthy plants (A), drought stressed plants (B), or plants challenged by fungal leaf spots (C), and from peanut A13 immature seeds without stress (D), drought stressed (E), or challenged by fungus Aspergillus flavus under drought stress (D). Genes examined: 1-Histone H3.3 (positive internal control), 2-Allergen Ara h3/4, 3-10-kDa protein, 4-Leucine-rich protein, 5-Bax inhibitor, 6-Heat shock cognate protein, 7-Non-specific lipid transfer protein, 8-drought inducible 22-kDa protein, 9-drought inducible RPR 10, 10-Defensin protein.