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Published online 1 January 2005
Published in Crop Sci 45:340-345 (2005)
© 2005 Crop Science Society of America
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Mapping Late Blight Resistance in Solanum microdontum Bitter

D. A. Bisognina, D. S. Douchesb,*, L. Buszkab, G. Bryanc and D. Wangb

a Dep. of Fitotecnia, Federal Univ. of Santa Maria, Santa Maria, RS, 97105-900, Brazil
b Dep. of Crop and Soil Sciences, Michigan State Univ., East Lansing, MI, 48824
c Scottish Crop Research Institute, Invergowrie, Dundee, Scotland


Figure 1
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  		Fig. 1. Quantitative trait loci associated with foliar late blight (LB) resistance of a S. microdontum-derived population on linkage group 21 for 1999, 2000, and joint analysis. Primer sequences of the SSR marker ST3334 were published by Ashkenazi et al. (2001), Stm by Milbourne et al. (1998) and ST13ST by Sandbrink et al. (2000).

 

Figure 2
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  		Fig. 2. Negative image of the simple sequence repeat marker Stm0020 fragments separated in 3% (w/v) Metaphor agarose gel run at 100 V for 4.5 h. A) S. microdontum (resistant parent), MSA133-57 (susceptible parent), bulks and ten resistant and susceptible progeny clones, respectively. Stm0020b linked with resistance and Stm0020a linked with susceptibility, both from S. microdontum. B and C) SSR marker Stm0020b followed through polyploidization. Parents of the diploid population, diploid resistant clone of the progeny (DLB1-150) and tetraploid parents with respective tetraploid progeny clones.

 




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