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Published online 1 January 2005
Published in Crop Sci 45:274-281 (2005)
© 2005 Crop Science Society of America
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Reduction of Ploidy Level by Androgenesis in Intergeneric Lolium–Festuca Hybrids for Turf Grass Breeding

David Kopeckya, Adam J. Lukaszewskib,* and Victor Gibeaultb

a Institute of Experimental Botany, Sokolovska 6, 77200 Olomouc, Czech Republic
b Dep. of Botany and Plant Sciences, Univ. of California, Riverside, CA 92521 USA


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  		Fig. 1. A–C. Regeneration of androgenic progeny for anthers of Lolium x Festuca hybrids. A. Multiple embryos formed in a burst anther of FpLp. B. Mature embryos and first plantlets of LmFp formed on the induction medium. C. Regeneration of a plantlet FpLp from an isolated embryo on the differentiation medium. D–H. In situ probing with total genomic DNA among the regenerants. In all instances, DNA of F. pratensis was used as a probe; chromatin of L. multiflorum was counterstained with propidium iodide D. Doubled haploid of L. multiflorum x F. pratensis with 14 pairs of chromosomes with identical patterns of the parental chromatin. E. 27-chromosome aneuploid of L. multiflorum x F. pratensis: 13 pairs of chromosomes and one single chromosome (arrowed). F. A haploid (14 chromosomes) of F. pratensis x L. perenne. Note a lower level of genome recombination than in the L. multiflorum x F. pratensis hybrid: four complete chromosomes of F. pratensis, three of L. multiflorum and seven translocations with a total of eight translocation breakpoints. G and H: two haploid members of a set of four plants recovered from what appeared to be a single embryo. These two plants have chromosomes with identical patterns of parental chromatin (the same letters on each photo marks chromosomes with the same pattern).

 




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