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RAPD Marker Variation among Divergent Selections for Fiber Concentration in Smooth Bromegrass

^a Dep. of Agronomy, Univ. of Wisconsin-Madison, WI 53706-1597 USA
^b USDA-ARS, U.S. Dairy Forage Research Center, 1925 Linden Dr. West, Madison, WI 53706-1108

View larger version (23K): [in a new window]   Fig. 1. Linear regressions of mean neutral detergent fiber (NDF) concentration on selection cycle for four smooth bromegrass populations divergently selected for NDF. Regressions statistics for Alpha, WB19e, Lincoln, and WB88S, respectively, were b = 7.2 ± 1.5, 7.0 ± 2.1, 6.6 ± 1.6, and 8.6 ± 1.4 g kg–1 cycle–1; R2 = 0.83, 0.79, 0.91, and 0.88.

View larger version (15K): [in a new window]   Fig. 2. Cluster dendrogram of 20 smooth bromegrass populations based on 83 RAPD marker band frequencies. Populations are abbreviated as follows: A = Alpha, B = WB19e, L = Lincoln, S = WB88S, 0 = original population, 1 = Cycle 1, 2 = Cycle 2, "+" = high NDF, and "-" = low NDF.

View larger version (23K): [in a new window]   Fig. 3. Scatterplot of genetic distances between paired smooth bromegrass populations that differ by one, two, three, or four selection cycles for divergent NDF concentration (enumerative difference between cycles). The linear regression equation was D = 0.004 + 0.024N, where N = enumerative difference between selection cycles, R2 = 0.71, P < 0.0001.

View larger version (20K): [in a new window]   Fig. 4. Scatterplot of genetic distances between paired smooth bromegrass populations within each of five divergent-NDF selection cycles, including the original populations (Cycle 0). The quadratic regression equation was D = 0.0579 – 0.0078C + 0.0064C2, R2 = 0.43, P = 0.0005.

View larger version (23K): [in a new window]   Fig. 5. Logistic regressions of RAPD marker band AG14.0825 frequencies (Pij) as a function of cycle and direction of selection for neutral detergent fiber (NDF) concentration in four smooth bromegrass populations. The common-slope logistic regression equation was LN[Pij/(1 – Pij)] = 1.8603 – 0.4487di1 – 1.0557di2 – 0.6267di3 + 0.4620i, where dij = 0 (absence) or 1 (presence) for each population; j = 1 for Alpha, 2 for WB19e, or 3 for Lincoln; and i = cycle number.