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Organelle DNA Diversity among Buffalograsses from the Great Plains of North America Determined by cpDNA and mtDNA RFLPs

Dep. of Agronomy and Horticulture, Univ. of Nebraska, Lincoln, NE 68503

View larger version (14K): [in this window] [in a new window]   Fig. 1. UPGMA dendrogram of 56 buffalograsses and two outgroups based on analysis of 36 monomorphic and 189 polymorphic fragments from 6 cpDNA and 3 mtDNA sequences.

View larger version (64K): [in this window] [in a new window]   Fig. 2. CpDNA restriction fragments of buffalograss accessions and two outgroups, following amplification with primers psbC-trnS and digestion with restriction enzyme HaeIII.

View larger version (103K): [in this window] [in a new window]   Fig. 3. Verification of maternal inheritance of cpDNA in buffalograsses by using cpDNA PCR-RFLPs with primers psbC-trnS and HaeIII. The genotype names are as follows: (1) representative of male parents 28, 32B, 102, 234; (2) undigested PCR fragment with primers; (3) DP-2F as maternal parent; (4-8) half-sib progenies; and (St) standard DNA marker.