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Origin of the Blue-Aleurone Gene in Sebesta Blue Wheat Genetic Stocks and a Protocol for Its Use in Apomixis Screening

L. A. Morrisona, R. J. Metzgerb,* and A. J. Lukaszewskic

a Herbarium, Dep. of Botany and Plant Pathology, Oregon State Univ., Corvallis, OR 97331-2910
b Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR 97331-3002
c Dep. of Botany and Plant Sciences, University of California, Riverside, CA 92521



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Fig. 1. Sequential C-banding (left) and FISH (right) of SB-3. Chromosomes labeled T are translocations 4BS.4BL.4EL. Note the hybridization pattern on 4EL.

 


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Fig. 2. Sequential C-banding (left) and FISH (right) of SB-1. Chromosomes labeled T1 and T2 are two translocations of Th. ponticum segments into unidentified wheat chromosomes. Note that the hybridization pattern on T1 is the same as on 4BS.4BL.4EL in SB3.

 


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Fig. 3. Two cycles of screening to identify apomictic plants of wheat (white-seeded form shown here). Possible apomictic lines (Class 2, F2x generation) are selected in the 1st Cycle and confirmed in the 2nd Cycle (Class 4, F2y generation)—i.e., production of only white seed verifies somatic embryo development. Segregating plants for white and blue seed (Classes 1 and 3) verify fertilization by the male SB parent.

 





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