Crop Science
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Abstract Freely available
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Related articles in Crop Science
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Satyavathi, V. V.
Right arrow Articles by Rao, M. B.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Satyavathi, V. V.
Right arrow Articles by Rao, M. B.
Agricola
Right arrow Articles by Satyavathi, V. V.
Right arrow Articles by Rao, M. B.
Related Collections
Right arrow Cell Biology & Molecular Genetics
Right arrow Crop Genetics

Effects of Growth Regulators on In Vitro Plant Regeneration in Durum Wheat

V. V. Satyavathia, P. P. Jauharb,*, E. M. Eliasa and M. B. Raoa

a Dep. of Statistics, North Dakota State Univ., Fargo, ND 58105
b USDA-ARS, Northern Crop Science Lab., Fargo, ND 58105



View larger version (125K):

[in a new window]
  		Fig. 1. Callus formation and plantlet induction in durum wheat cultivars. (A) Scutella showing calli from cultivar Lebsock, 3 wk after culture on modified Murashige and Skoog (MS) medium containing 2.0 mg L–1 dicamba. (B) Callus showing shoot buds of cultivar Maier, 4 wk after culture on medium containing 2.0 mg L–1 2,4-D (green shoot-like structures developed after exposure to light), and (C) 2.0 mg L–1 dicamba (green shoots developed in the dark. (D) Plantlet regeneration from cultivar Maier on MS basal medium from callus initiated on 2.0 mg L–1 dicamba.

 


View larger version (22K):

[in a new window]
  		Fig. 2. Effects of (A) the cultivar, (B) growth regulator, and (C) the concentration of the growth regulator on proportion of plants regenerated.

 


View larger version (61K):

[in a new window]
  		Fig. 3. Somatic metaphase chromosomes from root-tip cells and fluorescent genomic in situ hybridization of Maier regenerants from callus obtained on 2.0 mg L–1 dicamba. (A) 28 somatic chromosomes; (B) A 28-chromosome cell counterstained with propidium iodide (PI); and (C) Same cell as (B) hybridized with total genomic DNA of Triticum urartu and detected with fluorescein isothiocyanate (FITC). Brightly lit chromosomes belong to the A genome, while the faded chromosomes are from the B genome.

 




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Agronomy Journal Vadose Zone Journal
Journal of Natural Resources
and Life Sciences Education		 Soil Science Society of America Journal
Journal of Plant Registrations Journal of
Environmental Quality		 The Plant Genome
Copyright © 2004 by the Crop Science Society of America.