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Screening Multiple Soybean Cultivars (MG 00 to MG VIII) for Somatic Embryogenesis Following Agrobacterium-Mediated Transformation of Immature Cotyledons

^a Dep. of Natural Resources and Environmental Sciences, Univ. of Illinois, Urbana, IL 61801
^b USDA-ARS, Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, Dep. of Crop Sciences, Univ. of Illinois, Urbana, IL 61801

View larger version (53K): [in a new window]   Fig. 1. Somatic embryogenesis on the abaxial side of immature cotyledons under hygromycin selection following A. tumefaciens strain KYRT1-mediated transformation. A, B, and C, somatic embryos formed singly, in clusters, or as polyembryogenic structures, respectively. Histochemical GUS staining of responding explants are shown in (A) and (B). D, an opaque-type globular somatic embryo. E, a fused-type structure of translucent- and opaque-type SEs indicated by arrows. F, proliferative embryogenic tissues derived from an opaque-type SE.

View larger version (13K): [in a new window]   Fig. 2. Relationship between the embryogenic potential and transformation potential of 15 soybean cultivars in the presence of Agrobacterium and selective agents. The embryogenic potential of each cultivar was expressed as the estimated number of hygromycin-resistant SEs per 100 explants (% responding explant x no. of SEs per responding explant). The transformation potential of each of cultivar was expressed as the estimated number of GUS-positive SEs (no. of hygromycin-resistant SEs x % transformation frequency). Values for percent responding explant and % transformation frequency are shown in Table 1.

View larger version (127K): [in a new window]   Fig. 3. Representative explants showing three different phenotypes based on degree of cellular response and browning/necrosis along the margins of cotyledonary explants. These were classified as type-A (A), type-B (B), and type-C (C). Nonembryogenic callus formation with or without somatic embryogenesis under hygromycin selection was observed in many genotypes (D).