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Development of DNA Markers Suitable for Marker Assisted Selection of Three Pi Genes Conferring Resistance to Multiple Pyricularia grisea Pathotypes

Robert Fjellstroma,*, Concetta A. Conaway-Bormansb, Anna M. McClunga, Marco A. Marchettia, A. Robert Shanka and William D. Parkb

a USDA-ARS, Rice Research, 1509 Aggie Drive, Beaumont, TX 77713, USA
b Dep. of Biochemistry and Biophysics, Texas A&M Univ., College Station, TX 77843



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Fig. 1. Image of amplification products resulting from the Pibdom primers in selected rice cultivars commonly grown in U.S. producer fields electrophoresed on a nondenaturing polyacrylamide gel. Lane identities are as follows: Lanes 1 and 20, molecular weight marker; Lanes 2 to 19, rice cultivars Bengal, BL-1, Bolivar, Cocodrie, Cypress, Francis, Gulfmont, Hildalgo, Jasmine 85, Katy, L-204, Maybelle, M-202, M-205, Saber, Te-Qing, and Wells. Note that BL-1, Bolivar, Saber, and Te-Qing are the only cultivars displaying the 365-bp amplification product found in the Pi-b gene sequence, with its position shown by an arrow to the left of the image.

 


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Fig. 2. Genetic maps of marker loci on rice chromosomes 2, 11, and 12, respectively, near the (A) Pi-b gene from Te-Qing using combined data of crosses segregating for resistance to rice blast race IE-1K and presence of the Pibdom gene marker; (B) Pi-k locus conferring resistance to rice blast races IB-45, IB-54, or IG-1 using combined data of Pi-kh and Pi-ks allele segregation; and (C) Pi-ta2 gene from Katy or Kaybonnet using combined data of crosses segregating for resistance to rice blast race IB-49. Genetic distances in Morgan map units of recombination are displayed on left of each chromosome, with the centromere of chromosome 12 shown at its expected position.

 





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