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Influence of Bt Transgenes on Cell Wall Lignification and Digestibility of Maize Stover for Silage

H. G. Junga,* and C. C. Sheafferb

a USDA-ARS Plant Science Res. Unit and U.S. Dairy Forage Res. Center Cluster, Dep. of Agronomy and Plant Genetics, 411 Borlaug Hall, 1991 Upper Buford Circle, Univ. of Minnesota, St. Paul, MN 55108
b Dep. of Agronomy and Plant Genetics, 411 Borlaug Hall, 1991 Upper Buford Circle, Univ. of Minnesota, St. Paul, MN 55108



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Fig. 1. Whole maize plant moisture content and yield at four test locations in Minnesota during 2002. Data were averaged across 12 maize hybrids and four plots per location.

 


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Fig. 2. Acid detergent (ADL), Klason (KL), and acetyl bromide (AcBr) lignin concentration estimates for whole maize plants at four test locations in Minnesota during 2002. Data were averaged across 12 maize hybrids and four plots per location.

 


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Fig. 3. Whole maize plant crude protein (CP), starch, and neutral detergent fiber (NDF) concentrations at four test locations in Minnesota during 2002. Data were averaged across 12 maize hybrids and four plots per location.

 


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Fig. 4. Whole maize plant 24- and 96-h in vitro NDF digestibility at four test locations in Minnesota during 2002. Data were averaged across 12 maize hybrids and four plots per location.

 


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Fig. 5. Absorbance at 280 nm for an isolated maize stover lignin, alkali lignin (catalog number 37095-9; Aldrich, Milwaukee, WI), and ferulic acid (FA) in the acetyl bromide lignin method using the procedure of Hatfield et al. (1999).

 





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