HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in Crop Science Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Zhang, W. Articles by Takeda, K. Search for Related Content PubMed Articles by Zhang, W. Articles by Takeda, K. Agricola Articles by Zhang, W. Articles by Takeda, K. Related Collections Other Grain Crops Plant Genetic Resources

Differentiation of ß-Amylase Phenotypes in Cultivated Barley

^a Shijiazhuang Institute of Agricultural Modernization, Chinese Academy of Sciences, 286 Huaizhong Road, Shijia-zhuang, Hebei 050021, China
^b Plant Bioeng. Res. Lab. Sapporo Brew. Ltd., 37-1, Kizaki, Nitta, Gunma 370-0393, Japan
^c Research Institute for Bioresources, Okayama Univ., Chuo 2-20-1, Kurashiki, Okayama 710-0046, Japan

View larger version (12K): [in a new window]   Fig. 1. Frequency distribution of ß-amylase thermostability types in cultivated barley (n = 8263). Relative remaining activity after heat-treatment at 57.5°C for 30 min: A+: 68%; A: 58–62%; A–B:38–47% (OUI462 and OUI429, respectively); B: 25–35%; C: 1–5%.

View larger version (107K): [in a new window]   Fig. 2. Zymograms of ß-amylase in cultivated barley. Isoelectric focusing was done with a Phastsystem (Pharmcia, Sweden) and ready-made acrylamide gel (PhastGEL IEF 4.0-6.5; Amersham Bioscience, Uppsala, Sweden). Lane 1: Harrington; 2: OUNI096; 3: OUC1152; 4: Haruna Nijo; 5: OUI429; 6: OUI462; 7: OUU421.

View larger version (54K): [in a new window]   Fig. 3. Distribution of five major ß-amylase types in the world.