HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in Crop Science Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (12) Citing Articles via Google Scholar Google Scholar Articles by Adhikari, T. B. Articles by Goodwin, S. B. Search for Related Content PubMed Articles by Adhikari, T. B. Articles by Goodwin, S. B. Agricola Articles by Adhikari, T. B. Articles by Goodwin, S. B. Related Collections Wheat Plant Disease Crop Genetics

Microsatellite Markers Linked to the Stb2 and Stb3 Genes for Resistance to Septoria Tritici Blotch in Wheat

^a Department of Plant Pathology, 306 Walster Hall, North Dakota State University, Fargo, ND 58105-5012
^b USDA-ARS, Crop Production and Pest Control Research, Department of Botany and Plant Pathology, 915 West State Street, Purdue University, West Lafayette, IN 47907-2054
^c Cooperative Research Centre for Molecular Plant Breeding, South Australian Research and Development Institute (SARDI), GPO Box 397, Adelaide, SA 5001, Australia

View larger version (19K): [in a new window]   Fig. 1. Segregation of specific resistance to an Australian isolate of M. graminicola in the DH progeny developed from a cross between Veranopolis (containing the Stb2 gene for resistance) and the susceptible parent RAC875-2. Histograms showing frequency distribution of (A) percent leaf area covered with lesions of Septoria tritici blotch and (B) pycnidial density among 106 DH lines. Susceptible classes are indicated with horizontal lines. Phenotypic values of the parents are shown by arrows.

View larger version (18K): [in a new window]   Fig. 2. Segregation of specific resistance to an Australian isolate of M. graminicola in the DH progeny developed from a cross between Israel 493 (with the Stb3 gene for resistance) and the susceptible parent RAC875-2. Histograms showing frequency distribution of (A) percent leaf area covered with lesions of Septoria tritici blotch and (B) pycnidial density among 97 DH lines. Susceptible classes are indicated with horizontal lines. Phenotypic values of the parents are shown by arrows.

View larger version (37K): [in a new window]   Fig. 3. DNA bands amplified from parents and nine DH progeny derived from a cross between the resistant wheat cultivar Veranopolis (containing the Stb2 gene for resistance to M. graminicola) and the susceptible parent RAC875-2 with microsatellite primer pair Xgwm389 shown in a 3% agarose gel. A 25-bp DNA ladder was used as a standard size marker. Resistant and susceptible progeny are indicated by R and S, respectively. The 125-bp DNA fragment amplified from the resistant parent Veranopolis and resistant lines is indicated by the arrow on the left.

View larger version (20K): [in a new window]   Fig. 4. Molecular map of the short arm of wheat chromosome 3B showing the genetic location of the Septoria tritici blotch resistance gene Stb2. Markers were mapped in the 106 DH lines developed from a cross between the resistant cultivar Veranopolis (which carries the Stb2 gene for resistance to M. graminicola) and the susceptible parent RAC875-2. Approximate distances in centimorgans (cM) are indicated on the left; molecular markers and the resistance locus are on the right. The letter X in front of each SSR locus name indicates the basic symbol for a molecular marker with unknown function in wheat. Map distances are proportional to the distances in cM.

View larger version (39K): [in a new window]   Fig. 5. Segregation of amplification products of microsatellite locus Xgdm132 in a silver-stained polyacrylamide gel (12%) after polymerase chain reaction. DNA of 97 DH lines from a cross between the resistant wheat cultivar Israel 493 (P1) and the susceptible line RAC875-2 (P2) was evaluated; R = resistant, and S = susceptible to an Australian isolate of the Septoria tritici blotch pathogen, M. graminicola. A 25-bp DNA ladder was used as a standard size marker. The 150-bp DNA fragment amplified from the resistant parent Israel 493 and resistant lines is indicated by the arrow on the right. Two larger bands also amplified in each progeny line but gave the same pattern as the 150-bp band, so were considered to represent the same allele.

View larger version (20K): [in a new window]   Fig. 6. Locations of resistance genes and common microsatellite markers redrawn from different genetic maps of the distal region of wheat chromosome 3BS. The stem rust resistance gene Sr2 (A) was mapped by Spielmeyer et al. (2003). A major QTL for resistance to Fusarium head scab (Qfhs) was mapped by (B) Liu and Anderson (2003) and (C) Buerstmayr et al. (2003). The Septoria tritici blotch resistance gene Stb2 (D) was mapped in the present study. Genetic distances in cM are indicated to the left of the respective maps for those studies in which they were reported. Approximate locations of the Lr27 gene for resistance to leaf rust and a gene for phenylalanine ammonia lyase (PAL) from Faris et al. (1999), plus a major QTL for resistance to Stagonospora nodorum glume blotch (QSng) from Schnurbusch et al. (2003)(personal communication), are indicated on the far right. Common markers in different maps are joined by dashed lines.