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Identification of Microsatellite Markers Associated with a Stem Solidness Locus in Wheat

J. P. Cooka, D. M. Wichmanb, J. M. Martina, P. L. Brucknera and L. E. Talberta,*

a Department of Plant Sciences and Plant Pathology, Leon Johnson Hall, Montana State University, Bozeman, MT 59717
b Central Agric. Res. Center, HC90-Box 20, Moccasin, MT 59462



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Fig. 1. Histogram of stem solidness score for 93 doubled haploid lines from a solid-stemmed Rampart by hollow-stemmed Jerry cross. Solidness scores are combined means across three experimental locations.

 


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Fig. 2. Regression of stem solidness on molecular marker loci (Xgwm247, Xgwm340, and Xgwm547) determined by single marker linear regression QTL analysis. H = hollow marker allele contributed by Jerry, S = solid marker allele contributed by Rampart.

 


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Fig. 3. PCR amplified fragments from amplification of wheat genotypes with GWM340. Lanes 1 and 2 are winter wheat genotypes; three to six are spring wheat genotypes; seven is a pUC19/Rsa DNA ladder. Jerry and McNeal are hollow stemmed; Rampart, Rescue, Fortuna, and MT 9929 are solid stem genotypes.

 





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