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RAPD-Based Genetic Relationships in Kentucky Bluegrass

Comparison of Cultivars, Interspecific Hybrids, and Plant Introductions

Dep. of Plant Pathology, Univ. of Wisconsin, 1630 Linden Dr., Madison, WI 53706

View larger version (16K): [in a new window]   Fig. 1. Multidimensional scaling plot of the genetic distance matrix computed from RAPD data generated from 123 Kentucky bluegrass genotypes, classified into commercial cultivars, plant introductions, experimental breeding lines, and interspecific (Poa pratensis x P. arachnifera) hybrids, shown as (TX-KY).

View larger version (19K): [in a new window]   Fig. 2. Multidimensional scaling plot of the genetic distance matrix computed from RAPD data generated from all 85 Kentucky bluegrass cultivars, classified into 12 morphological trait-based groups, each represented by different symbols. Also included are the 13 experimental breeding lines.

View larger version (116K): [in a new window]   Fig. 3. RAPD-derived gel profile, using Operon primer AF20, of three seedling replicates, labeled ‘1’, ‘2’, and ‘3’, of Kentucky bluegrass cultivars Nassau and Chateau. The arrows represent bands amplified in replicate 1 of cultivar Nassau but not in replicates 2 or 3.