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Identification of Molecular Markers Linked to the Fertility Restorer Genes for CMS-D8 in Cotton

^a Dep. of Agronomy and Horticulture, New Mexico State Univ., Las Cruces, NM 88003
^b Dep. of Crop, Soil, and Environmental Sci., Univ. of Arkansas, Fayetteville, AR 72701

View larger version (15K): [in a new window]   Fig. 1. Polymerase chain reaction product profile of DNA amplified with primer UBC188 in Population 2: (D8R x TM-1) F1 x TM1. Lanes labeled "F" and "S" represent individual fertile and sterile plants, respectively. Lane "M" is a 100-bp ladder. The arrow indicates the RAPD marker, UBC188500, which was present only in the fertile plants.

View larger version (50K): [in a new window]   Fig. 2. Random amplified polymorphic DNA profile of DNA amplified with primer UBC169 in Population 5: (D2R x ARK8518)F1 x ARK8518. Lanes labeled "S" and "F" represent sterile and fertile plants, respectively. Lane M is 100-bp ladder. The arrow shows an extra band (UBC169700) in fertile plants.

View larger version (16K): [in a new window]   Fig. 3. A consensus map comprising Rf1, Rf2, and four DNA markers. Rf1 is either (a) flanked by Rf2 and UBC1113000, or (b) flanked by Rf2 and UBC188500.