HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in Crop Science Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Haen, K. M. Articles by Faris, J. D. Search for Related Content PubMed Articles by Haen, K. M. Articles by Faris, J. D. Agricola Articles by Haen, K. M. Articles by Faris, J. D. Related Collections Wheat Cell Biology & Molecular Genetics Crop Genetics

Genomic Targeting and High-Resolution Mapping of the Tsn1 Gene in Wheat

^a Department of Plant Sciences, Loftsgard Hall, North Dakota State University, Fargo, ND 58105
^b USDA-ARS, Cereals Crops Research Unit, Northern Crop Science Laboratory, Fargo, ND 58105

View larger version (59K): [in a new window]   Fig. 1. Reaction to infiltration of Ptr ToxA into leaves of insensitive (top) and sensitive (bottom) wheat genotypes. The insensitive genotype was W7976 and the sensitive genotype was Kulm.

View larger version (39K): [in a new window]   Fig. 2. Flow chart outlining the scheme for targeting subgenomic regions in wheat using bulked segregant analysis. DNA of the two bulks are compared by AFLP or cDNA-AFLP analysis. Positive fragments are cloned and used as RFLP probes to detect fragments on Southern blots containing Chinese Spring (CS), the nullisomic-tetrasomic stocks involving homeologous group 5 chromosomes, and appropriate 5BL chromosome deletion lines to verify the existence of the fragment in the targeted interval. The marker was then placed on the physical map of the corresponding chromosome. The probe was also mapped in segregating populations to generate genetic linkage maps. The genetic maps were compared with the corresponding deletion intervals of the physical map to reveal marker order and estimate physical to genetic distance ratios.

View larger version (27K): [in a new window]   Fig. 3. Comparison of a portion of the 5BL physical map (left) with low-resolution genetic maps of the CS x CS-DIC 5B RSL population (middle) and the LDN x LDN-DIC 5B (Israel A) BC population (right) corresponding to the 5BL-14/5BL-16 deletion interval. On the physical map, fraction breakpoint values and corresponding deletion line names are given on the left, and marker names and their physical deletion interval positions are on the right. For the genetic maps, centimorgan distances are indicated on the left, and marker names and their relative genetic positions are given on the right. Markers shown in bold were generated by the genomic targeting scheme. Markers shown in plain text are RFLP markers mapped by Faris et al. (2000). Tsn1 lies in the physical segment corresponding to the 5BL-14/5BL-9 deletion interval, close to the 5BL-9 breakpoint (see Fig. 4). Tsn1 is not shown on the CS x CS-DIC 5B RSL map because this population does not segregate for reaction to Ptr ToxA.

View larger version (18K): [in a new window]   Fig. 4. High-resolution genetic maps of the Tsn1 region generated in 633 F2s derived from W7976 x Kulm (left) and 930 F2s derived from LDN x LDN-DIC 5B (PI478742) (right). Markers are indicated to the right of the maps and centimorgan distances to the left. The physical breakpoint of deletion line 5BL-9 is indicated between Xfcg9 and Xfcg10.