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QTL Analysis of Morphological, Developmental, and Winter Hardiness-Associated Traits in Perennial Ryegrass

^a National Agricultural Research Center for Hokkaido Region, National Agricultural Research Organization, Hitsujigaoka, Sapporo, 062-8555, Japan
^b Crop Genetics Research and Development, Pioneer Hi-Bred International, 7300 NW 62nd Avenue, Johnston, IA 50131-1004
^c Plant Biotechnology Centre, Primary Industries Research Victoria, Department of Primary Industries, La Trobe University, Bundoora, Victoria 3086, Australia and Cooperative Research Centre for Molecular Plant Breeding, Australia
^d Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Sapporo, 060-8589, Japan
^e Pastoral and Veterinary Institute, Primary Industries Research Victoria, Department of Primary Industries, Private Bag 105, Hamilton, Victoria 3300, Australia and Cooperative Research Centre for Molecular Plant Breeding, Australia
^f Rhydgoch Genetics, Rhydhir Uchaf, Comins Coch, Aberystwyth, Ceredigion SY23 3BJ, Wales, UK

View larger version (64K): [in a new window]   Fig. 1. Frequency distribution bar-charts for traits associated with (A) vegetative morphogenesis, (B) reproductive morphogenesis and development and (C) winter hardiness measured in the progeny set of the p150/112 reference genetic mapping population in perennial ryegrass. Phenotypic scores have been assigned to intervals on the y = 0 axis up to the maximum value indicated for each interval. The mean score of the heterozygous p150/112 parent is indicated in the relevant interval by an arrow, with the appropriate numerical value. The doubled haploid parent was not available for phenotypic analysis.

View larger version (44K): [in a new window]   Fig. 2. Location of QTLs for morphological traits, developmental traits and electrical conductivity on the SSRP, RFLP and AFLP-based reference genetic map of perennial ryegrass, based on the results of simple interval mapping (SIM). The maximum likelihood position of the QTL is indicated with an arrow. Bar and line lengths indicate a LOD drop of 1.0 and 2.0 respectively on either side of the maximum likelihood position.