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Rapid Iodine Staining Techniques for Identifying the Waxy Phenotype in Sorghum Grain and Waxy Genotype in Sorghum Pollen

^a USDA-ARS, NPA Wheat, Sorghum and Forage Research, 344 Keim Hall, University of Nebraska-Lincoln, Lincoln, NE 68583-0937
^b USDA-ARS, NPA, GMPRC, 1515 College Avenue, Manhattan, KS, 66502

View larger version (115K): [in a new window]   Fig. 1. Iodine staining of crushed and gelatinized individual sorghum seeds. One genotype per column, one seed per cell. Genotypes in Columns 1 through 8, respectively, are: BTx630, TxAGR1, RTx430, Wheatland, B9307, B94C274, Redlan, RTx2907.

View larger version (84K): [in a new window]   Fig. 2. Iodine staining of fresh pollen magnified 40x. BTx630 (top), BTxAGR1 (middle), and RTx430 (bottom).

View larger version (151K): [in a new window]   Fig. 3. Iodine staining of 30-d-old pollen magnified 40x. BTx630, RTx430, Wheatland (from top to bottom, left column), BTx623, Redlan (from top to bottom, right column).