HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in Crop Science Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Tang, J. Articles by McVetty, P. B. E. Search for Related Content PubMed Articles by Tang, J. Articles by McVetty, P. B. E. Agricola Articles by Tang, J. Articles by McVetty, P. B. E. Related Collections Cell Biology & Molecular Genetics Crop Genetics Other Oil Crops

Stability of the Expression of Acyl-ACP Thioesterase Transgenes in Oilseed Rape Doubled Haploid Lines

Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

View larger version (54K): [in a new window]   Fig. 1. PCR analyses for the three DH1 plants that were developed from embryos selected with kanamycin and did not accumulate lauric acid in the seed oil. a. PCR with the bay-TE gene primers for a 1.0-kb fragment and the napin promoter primers for a 0.5-kb control band. b. PCR with the nptII gene primers for a 0.7-kb fragment and the napin promoter primers. Lanes 1 to 3, the three DH1 plants; Lane 4, the bay-TE transgenic parental line TL1; Lane 5, nontransgenic control. Sizes of DNA molecular weight markers (L) are indicated in base pair.

View larger version (31K): [in a new window]   Fig. 2. PCR analyses using the nptII gene primers for a 0.7-kb fragment and the napin promoter primers for a 0.5-kb control band for the 17 oilseed rape DH1 plants that were developed from embryos selected with kanamycin and did not show enhanced levels of palmitic acid in the seed oil. Lanes 1 to 17, the 17 DH1 plants; Lane 18, the cuphea-TE transgenic parental line TL6; Lane 19, nontransgenic control plants. Sizes of DNA molecular weight markers (L) are indicated in base pair (bp).

View larger version (28K): [in a new window]   Fig. 3. PCR analyses using the cuphea-TE primers for the amplification of a 1.1-kb fragment and the napin promoter primers for a 0.5-kb control band for the nine oilseed rape DH1 plants that showed the nptII gene. Lanes 1 to 17, the nine DH1 plants with the same lane number as in Fig. 2; Lane 18, the cuphea-TE transgenic oilseed rape parental line TL6; Lane 19, nontransgenic control. Sizes of DNA molecular weight markers (L) are indicated in base pair (bp).

View larger version (35K): [in a new window]   Fig. 4. Southern blotting analyses of oilseed rape cuphea-TE transgenic parental line TL6 and DH2 seedlings originating from eight DH1 plants by probing with the cuphea-TE transgene probe (a) followed by re-probing with the nptII gene probe (b) and schematic representation of the position of the probes in the T-DNA construct, not drawn to scale (c). The estimated sizes of the bands are shown in kilobase pair (kb). Lanes 1 to 8 represent the eight DH1 plants that were developed from crosses of the cuphea-TE transgenic parental line TL6 with nontransgenic plants and showed cuphea-TE expression by enhanced levels of palmitic acid (18.7–35.5% palmitic acid) in DH2 seed oil; P, a plasmid carrying the cuphea-TE transgene; CK, nontransgenic control plants. LB, left T-DNA border; RB, right T-DNA border; 35S, CaMV 35S promoter; tml, tml 3' region; nptII, neomycin phosphotransferase gene; napin 5', a seed specific promoter of the napin gene from B. rapa; napin 3', 3' termination fragment of the napin gene; cuphea-TE, the cuphea-TE transgene. Hatched bars represent the probes for the nptII gene and the cuphea-TE transgenes. The NsiI site is approximately 1.9 kb from RB and 5.9 kb from LB (Jones et al.., 1995; Kridl et al.., 1991; McBride and Summerfelt, 1990).

View larger version (18K): [in a new window]   Fig. 5. Lauric acid (C12:0) or palmitic acid (C16:0) level (%) in the seed oil of individual DH3 plants of eight oilseed rape DH lines carrying the bay-TE or the cuphea-TE transgenes. Each dot represents the average of two tests of seed samples from the same plant.