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Molecular Diversity of a Germplasm Collection of Squash (Cucurbita moschata) Determined by SRAP and AFLP Markers

Center for Conservation and Breeding of the Agricultural Diversity (COMAV), Polytechnic University of Valencia, Camino de Vera 14, Valencia 46022, Spain

View larger version (18K): [in a new window]   Fig. 1. a. Diagram showing the relationships among the quantitative characters used for the characterization of 47 accessions of C. moschata based on PCA. b. Diagram showing the relationships among 47 accessions of C. moschata based on PCA using morphological characterization. Flattened (); Globular (); Round (•); Oval (*); Oviform (); Heart-shaped (); Cuneiform (); Oblong (); Cylindrical (); Pear-shaped (); Dumbbell (); Butternut (); Guiro (+); Unclassified (°). Accessions used for AFLP analysis.

View larger version (126K): [in a new window]   Fig. 2. Example of molecular diversity of some accessions of C. moschata determined by SRAP markers. The lane in the left end shows a negative control and the lane in the right end is a molecular size marker (Marker VI, Roche).

View larger version (28K): [in a new window]   Fig. 3. Dendrogram showing the relationships among 47 accessions of C. moschata with SRAP markers on the basis of DICE Distance (Nei and Li, 1979) and UPGMA. Bootstrap values are based on 1000 resamplings of the data set and are indicated as a percentage. Only the bootstrap values over 50% are indicated.

View larger version (18K): [in a new window]   Fig. 4. Diagram showing the relationships among 47 accessions of C. moschata based on PCoA with SRAP markers. Flattened (); Globular (); Round (•); Oval (*); Oviform (); Heart-shaped (); Cuneiform (); Oblong (); Cylindrical (); Pear-shaped (); Dumbbell (); Butternut (); Guiro (+); Unclassified (°).

View larger version (27K): [in a new window]   Fig. 5. Dendrogram showing the relationships among 39 accessions of C. moschata with AFLP markers on the basis of DICE Distance (Nei and Li, 1979) and UPGMA. Bootstrap values are based on 1000 resamplings of the data set and are indicated as a percentage. Only the bootstrap values over 50% are indicated.

View larger version (16K): [in a new window]   Fig. 6. Diagram showing the relationships among 39 accessions of C. moschata based on PCoA with AFLP markers. Flattened (); Globular (); Round (•); Oval (*); Oviform (); Heart-shaped (); Cuneiform (); Oblong (); Cylindrical (); Pear-shaped (); Dumbbell (); Butternut (); Guiro (+); Unclassified (°).