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Introgression in Common Bean x Tepary Bean Interspecific Congruity-Backcross Lines as Measured by AFLP Markers

^a International Center for Tropical Agriculture, Biotechnology Research Unit, CIAT, A.A. 6713 Cali, Colombia
^b International Potato Center, CIP, A.A. 1558 Lima 12, Peru

View larger version (94K): [in a new window]   Fig. 1. Amplified fragment length polymorphism (AFLP) analysis of 38 interspecific congruity backcross lines derived from the cross between common bean (Phaseolus vulgaris) cultivar ‘ICA Pijao’ (P.v.) and tepary bean (P. acutifolius) accession G40001 (P.a.) using the AFLP primer combination E-ACC/M-CTA. Arrows indicate polymorphic bands associated with introgression of tepary bean allele in a common bean background. A 25-bp ladder was used as a MW size standard.

View larger version (116K): [in a new window]   Fig. 2. Amplified fragment length polymorphism (AFLP) analysis of common bean lines (Phaseolus vulgaris/P.v.); tepary bean accessions (P. acutifolius/P.a.) and interspecific hybrid advanced lines (series SEL, VAX, and XAN) or cultivars (Tara and Jules), as indicated by asterisk, using the primer combination E-ACC/M-CTA. A 25-bp ladder was used as a MW size standard.

View larger version (20K): [in a new window]   Fig. 3. Dendrogram showing the association between 58 Phaseolus genotypes: 4 tepary bean (P. acutifolius), 2 common bean (P. vulgaris), 14 recurrent backcross interspecific lines (SEL, VAX, and XAN) or cultivars (Tara, Jules) and 38 congruity backcross (CBC) interspecific hybrids. UPGMA analysis was based on genetic similarities calculated with the Dice coefficient (Nei and Li, 1979). The values above key nodes denote 95% confidence intervals for those nodes, as revealed by a bootstrap analysis.

View larger version (20K): [in a new window]   Fig. 4. Multiple correspondence analysis showing the association between 58 Phaseolus genotypes: 4 tepary beans (P. acutifolius), 2 common bean (P. vulgaris), 14 recurrent backcross interspecific lines (SEL, VAX, and XAN) or cultivars (Tara, Jules) and 38 congruity backcross (CBC) interspecific hybrids. Three clusters indicated with circles corresponding to I: genotypes of common beans and interspecific with low rates of introgression. II interspecific congruity backcross lines with higher rates of introgression and III genotypes of P. acutifolius. Arrows indicate the position of genotypes; ICA Pijao (P. vulgaris), XAN 159 (recurrent backcross interspecific line) and G40001 (P. acutifolius).