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Nitrate Uptake of Seedling and Mature Kentucky Bluegrass Plants

^a Dep. of Plant Sci., State Univ. of New York, College of Agriculture and Technology, Cobleskill, NY 12043
^b Dep. of Plant Sci., Univ. of Rhode Island, Kingston, RI 02881

View larger version (27K): [in a new window]   Fig. 1. Time course of seedling nitrate uptake of six Kentucky bluegrass cultivars: BL, Blacksburg; BZ, Barzan; CN, Conni; DN, Dawn; EC, Eclipse; GM, Gnome. Thirty days after seeding, six seedlings of uniform size from each cultivar were placed individually in polystyrene cups filled with 2 mL of a nutrient solution containing 0.6 mM nitrate, and the rate of nitrate uptake was monitored at 24-h intervals. On Days 5 and 19, deionized water was provided (indicated by "–NO3–") in place of the nitrate solution. Immediately before the start of nitrate uptake on Days 9 and 22, one-third of the leaf blade lengths were clipped off of each seedling (indicated by "1st cut" and "2nd cut"). On Day 10, no nitrate uptake but nitrate efflux from the roots was detected (indicated by "Efflux").

View larger version (61K): [in a new window]   Fig. 2. Comparisons of nitrate uptake rate (NUR) among seedlings within each of six Kentucky bluegrass cultivars: (A) Blacksburg, (B) Barzan, (C) Conni, (D) Dawn, (E) Eclipse, (F) Gnome. The mean NUR for each seedling was based on 19 daily observations shown in Fig. 1. The LSD at P < 0.05 was shown as the last bar in each graph.

View larger version (36K): [in a new window]   Fig. 3. Relationships between nitrate uptake rate (NUR) and morphological characteristics of Kentucky bluegrass seedlings. (A) relationship between NUR of Days 6 to 8 and total leaf lengths of the first clippings, (B) relationship between NUR of Days 6 to 8 and total leaf area of the first clippings, (C) relationship between NUR of Days 20 and 21 and total leaf length of the second clippings, (D) relationship between NUR of Days 20 and 21 and total leaf area of the second clippings, (E) relationship between NUR of Days 20 and 21 and total root length of the second clippings, (F) relationship between NUR of Days 20 and 21 and total root surface area of the second clippings.