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Dinitrogen Fixation in Illinois Bundleflower

Jaehyun Byuna, Craig C. Sheaffer*,a, Michael P. Russelleb, Nancy J. Ehlkea, Donald L. Wysea and Peter H. Grahamc

a Dep. of Agronomy and Plant Genetics, 411 Borlaug Hall, St. Paul, MN 55108
b USDA-ARS Plant Science Res. Unit and Dep. of Soil, Water, and Climate, 439 Borlaug Hall, St. Paul, MN 55108
c Dep. of Soil, Water, and Climate, 439 Borlaug Hall, Univ. of Minnesota, 1991 Upper Buford Circle, St. Paul, MN 55108



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Fig. 1. Dendrogram showing relationships among rhizobial isolates (1–89) from nodules on Illinois bundleflower at three Minnesota locations and Nitragin strains 43A1 and 43C2 based on rep-PCR (polymerase chain reaction) analysis. Vertical dashed line represents 70% similarity among strains.

 


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Fig. 2. Cumulative aboveground N in Illinois bundleflower derived from symbiotic N2 fixation at three Minnesota locations in 2 yr following seeding, based on three methods for estimating N2 fixation. Bars represent least squares means derived from six replicates of three accessions at each location. Error bars represent the maximum standard error of the least squares means for the three separate models comparing pairs of methods.

 


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Fig. 3. Reliance of Illinois bundleflower herbage on N derived from symbiotic N2 fixation at three Minnesota locations in 2 yr following seeding, based on three methods for estimating N2 fixation. Bars represent least squares means derived from six replicates of three accessions at each location. Error bars represent the maximum standard error of the least squares means for the three separate models comparing pairs of methods.

 





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