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Identification and Characterization of a Low Phytic Acid Wheat

^a Univ. of Idaho, Aberdeen Research and Extension Center, P.O. Box 870, Aberdeen, ID 83210
^b BASF Corp., Research Triangle Park, NC 27709
^c USDA-ARS Small Grains and Potato Research Unit, P.O. Box 607, Aberdeen, ID 83210

View larger version (39K): [in a new window]   Fig. 1. Js-12-LPA mutant phenotype. (A) Assay for high inorganic phosphate phenotype (HIP). Five kernels per plant were extracted individually and assayed to infer maternal genotype. Wild-type kernels had low inorganic P (low color development). Homozygous mutant kernels had high Pi, producing color. (B) HPLC assay of phytic acid in a single Js-12-WT kernel of wheat and a single Js-12-LPA kernel. Js-12-WT and Js-12-LPA phytic acid P levels were 3.8 and 2.5 mg g–1, respectively. Inositol phosphates of lower phosphorylation were not observed.

View larger version (30K): [in a new window]   Fig. 2. Distribution of genotypes assigned to F2 plants from the cross Js-12-LPA/IDO563. F2 plants were classified into five categories: (1) Homozygous wild-type if no progeny (F2:3) kernels were HIP (high inorganic phosphate) and all F2:3 kernels with intermediate Pi concentrations had Pi concentrations below the mid-parent mean; (2) Heterozygous if at least one F2:3 kernel was HIP and at least one F2:3 kernel was wild-type; (3) Heterozygous if HIP kernels were observed, no wild-type F2:3 kernels were observed, and kernels of intermediate Pi concentration had concentrations below the mid-parent mean; (4) Homozygous for the low phytic acid trait if no F2:3 kernels were wild-type and all F2:3 kernels with intermediate Pi concentrations had Pi concentrations above the mid-parent mean; (5) Homozygous for the low phytic acid trait if all F2:3 kernels were HIP.

View larger version (21K): [in a new window]   Fig. 3. Distribution of F4:6 families for inorganic P as a percentage of total P and phytic acid P as a percentage of total P.