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Reproductive and Molecular Evidence for Allogamy in Lotononis bainesii Baker

^a Forage Legume Department, National Institute of Agricultural Research, INIA Tacuarembó, Ruta 5 Km 386, Tacuarembó, Uruguay
^b Biotechnology Unit, National Institute of Agricultural Research, INIA Las Brujas, Ruta 48 km 10, Canelones, Uruguay
^c Dep. of Agronomy, Univ. of Florida, Gainesville, FL 32611-0500, USA

View larger version (126K): [in a new window]   Fig. 1. Flower of L. bainesii showing the stigma above of the anthers (a; arrow) and two pictures of the fruit (b and c); note the long hooked stylar tissue adhering to the pod.

View larger version (58K): [in a new window]   Fig. 2. Polymorphism shown by RAPD analysis, primer G-17, in parental material. The arrow indicates a fragment used for cloning and sequencing to develop the SCAR marker. The DNA ladder (Promega Corp., Madison, WI) on right consists of 11 fragments that range in size from 100 to 1000 bp in 100-bp increments, plus an additional fragment at 1500 bp. The 500-bp fragment, present at increased intensity, is indicated by an arrow.

View larger version (43K): [in a new window]   Fig. 3. Ten self-pollinated progenies and the mother plant on the right next to the ladder marker obtained with the SCAR II. The fragment with increased intensity in the DNA ladder (Promega Corp.) on the right of the gels is 500 bp.

View larger version (94K): [in a new window]   Fig. 4. SCAR electrophoretic pattern of 15 individual genotypes of three inflorescences (a, b, and c) from open pollinated flowers obtained with SCAR II primer. The tester genotype used as a mother plant has only one band. The fragment with increased intensity in the DNA ladder (Promega Corp.) on the right of the gels is 500 bp.

View larger version (63K): [in a new window]   Fig. 5. Analysis of CAPS markers utilizing the enzyme TaqI in an open pollinated progeny of a mother plant (M). The arrows indicate the non maternal allele present in the progeny.