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Development and Utilization of SSRs to Estimate the Degree of Genetic Relationships in a Collection of Pearl Millet Germplasm

^a 377 Plant Sci., Dep. of Agronomy and Horticulture, Univ. of Nebraska, Lincoln, NE 68583
^b Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705

View larger version (62K): [in a new window]   Fig. 1. Polymerase chain reaction amplification of pearl millet genomic DNA from 15 lines. Lanes: 1 = 81B, 2 = 94M59464B CBR, 3 = 293B, 4 = 51735B, 5 = 59025B, 6 = 086R1, 7 = Bmr eB, 8 = PI 164421, 9 = PI 286892, 10 = PI 536327, 11 = PI 307713, 12 = PI 511036, 13 = PI 561619, 14 = PI 583799, 15 = Tift 85DB, and lane M contains a 50-bp size marker (Promega Corp., Madison, WI). Two microsatellites, (A) PSM 2202 (Qi et al., 2001) and (B) CTM9, were assayed. The DNA samples were fractionated in 12% nondenaturing acrylamide gels stained with ethidum bromide.

View larger version (61K): [in a new window]   Fig. 2. Dendrogram of 53 pearl millet lines based on the unweighted pair-group method with arithmetic averages analysis using the similarity matrix generated by the Nei and Li coefficient after amplification with 30 pairs of microsatellite primers.