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Development of SCAR Marker Linked to a Major QTL for High Fiber Strength and Its Usage in Molecular-Marker Assisted Selection in Upland Cotton

Wangzhen Guoa, Tianzhen Zhang*,a, Xinlian Shena, John Z. Yub and Russell J. Kohelb

a National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Inst., Nanjing Agric. Univ., Nanjing 210095, China
b USDA ARS, Southern Plain Agriculture Research Center, Crop Germplasm Research Unit, College Station, TX 77845, USA



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  		Fig. 1. EcoRI digestion of the recombinant clones. Lane 1-2: EcoRI digested p757-1365; Lane 3: undigested p757-1365; Lane 4: EcoRI digested p431-1920; Lane 5: undigested p431-1920; and M: Lambda DNA/EcoRI + HindIII.

 


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  		Fig. 2. Development of SCAR markers for UBC7571365 and UBC4311920. Lanes 1, 2, 3, and 4 show products of 7235, TM-1, F1 and p757-1365 amplified with a pair of primer, P2F and P2R; lanes 5,6,7, and 8 show products of 7235, TM-1, F1 and p431-1920 amplified with a pair of primer, P1F and P1R; and M, Lambda DNA/EcoRI + HindIII.

 


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  		Fig. 3. The amplified results with SCAR4311920 in backcrossing/selfing breeding population M, Lambda DNA/EcoRI + HindIII. Lane 1-4 showed amplification product of 7235, TM-1, F1 and p431-1920; and lanes 5-87 represented different high strength individual plant from (SM 3x7235) BC1F4 families.

 


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  		Fig. 4. A chromosome 10 segment showing molecular markers linked to the major QTL for fiber strength in Upland cotton 7235.

 




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