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Estimation of Pollen Viability, Shedding Pattern, and Longevity of Creeping Bentgrass on Artificial Media

^a Dep. of Horticulture, 257 Horticulture Hall, Iowa State Univ., Ames, IA 50011
^b The Scotts Co., 14111 Scottslawn Rd., Marysville, OH 43041

View larger version (38K): [in a new window]   Fig. 1. The effect of sucrose concentration on pollen tube length (average of 30 germinating pollen for each treatment). Pollen grains were collected from Clone 7 of ‘Crenshaw’ bentgrass at 1000, 1100, 1200, and 1300 h, respectively. Columns within each pair having a same letter are not significantly different from each other at = 0.05. Bars indicate SE.

View larger version (62K): [in a new window]   Fig. 2. Germination of pollen from Clone 7 of ‘Crenshaw’ on medium containing (A) 0.5 M or (B) 1.0 M sucrose. The rest of the medium components are the same with 1 mM H3BO3, 2 mM CaCl2, and 0.3% (by weight) Phytogel. Note the short and thick pollen tubes in (A) vs. the long and slender pollen tube in (B). A germinating pollen of Clone 5 of ‘Penncross’ with double pollen tubes on a medium with 0.5 M sucrose, 1.0 mM H3BO3, 2.0 mM CaCl2, and 0.3% (by weight) Phytogel (C).

View larger version (30K): [in a new window]   Fig. 3. Pollen germination percentage across a period of 10 h during the day from 0800 h to 1700 h. Pollen grains were collected from Clone 9 of ‘Crenshaw’ creeping bentgrass and germinated at room temperatures on the medium containing 1.0 M sucrose, 1.0 mM H3BO3, 2.0 mM CaCl2, and 0.3% (by weight) Phytogel. Bars indicate SE.

View larger version (28K): [in a new window]   Fig. 4. Longevity of pollen grains collected from Clone 5 of ‘Penncross’ and Clone 9 of ‘Crenshaw’ between 1000 and 1100 h. Pollen were stored in a sealed desiccator with a relative humidity of 64 to 66% at 21°C. Pollen were removed from the desiccator every 20 min and germinated on the medium containing 1.0 M sucrose, 1.0 mM H3BO3, 2.0 mM CaCl2, and 0.3% (by weight) Phytogel at room temperatures. Bars indicate SE.