HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wang, J. P. Articles by Sleper, D. A. Search for Related Content PubMed Articles by Wang, J. P. Articles by Sleper, D. A. Agricola Articles by Wang, J. P. Articles by Sleper, D. A. Related Collections Turfgrass Crop Genetics

Genome Introgression of Festuca mairei into Lolium perenne Detected by SSR and RAPD Markers

^a Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824
^b Department of Agronomy, University of Missouri-Columbia, Columbia, MO 65211

View larger version (98K): [in a new window]   Fig. 1. The phenotypes of the plant materials. a. the parents (F. mairie and L. perenne) and F1 hybrid; b. the backcross progeny.

View larger version (50K): [in a new window]   Fig. 2. The SSR profiles detected by automated capillary electrophoresis of fluorochrome-labeled PCR products. a. A single monomorphic allele of 191 bp was amplified from all the parental genotypes by primer LPSSRK14B01, one of the three primer pairs that could not distinguish between species. b. The shared allele (246 bp) between the parents was amplified from the parents by LPSSRH11G05, which is one of the 4 primer pairs that could not fully discriminate Fm and Lp. c. The SSR pattern amplified from the primer pair LPSSRK11E11, that could fully discriminate Fm and Lp. The fragment sized 125 bp is the Fm specific allele and 108 bp is the Lp specific allele. d. The SSR profile amplified from the primer pair LPSSRK10F08, that could confirm the hybrid nature of F1 plants by presence of parental bands (Fm specific-alleles: 76 and 110 bp and Lp specific-alleles: 115 and 136 bp) and could also detect the segregation of alleles in backcross population.

View larger version (31K): [in a new window]   Fig. 3. The RAPD banding pattern (negative image) of F. mairei (lane 1), L. perenne (lanes 2 and 3), F1 hybrid between Fm and Lp1 (lane 4), and backcross progeny (lane 5-17). The whole arrows point to the Fm-specific bands (lane 1). The half arrow point to the new bands (lanes 8 and 15). M = 1-kb size ladder.