HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Chen, X. Articles by Dubcovsky, J. Search for Related Content PubMed Articles by Chen, X. Articles by Dubcovsky, J. Agricola Articles by Chen, X. Articles by Dubcovsky, J. Related Collections Crop Genetics Wheat Plant Disease

Development of Sequence Tagged Site and Cleaved Amplified Polymorphic Sequence Markers for Wheat Stripe Rust Resistance Gene Yr5

^a USDA-ARS and Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430
^b Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430
^c Department of Agronomy & Range Science, University of California, Davis, CA 95616

View larger version (59K): [in a new window]   Fig. 1. The sequences of the resistance gene analog polymorphism (RGAP) marker Xwgp-17 and Xwgp-18 and the positions of sequence tagged site (STS) primers. The six base pairs in the box were deleted and the underlined base pairs were substituted in Xwgp-18. The sequences of primer Yr5STS-12 and Yr5STS-13 are identical except for the 17th base pair based on different clones that were differed at that base position. The DpnII site (gatc) used for the CAPS is shown at the bp 394 to 397.

View larger version (50K): [in a new window]   Fig. 2. Silver stained polyacrylamide gel showing polymorphic bands associated with the Yr5 resistance. A: Codominant STS markers amplified with primers Yr5STS-7 (5'-GTACAATTCACCTAGAGT-3') and Yr5STS-8 (5'-GCAAGTTTTCTCCCTATT-3'). The higher band (478 bp) was present in the resistant parent (the Yr5 NIL) and the resistant BC7:F3 bulk and the lower band (472 bp) was present in the susceptible BC7:F3 bulk and the susceptible parent (AVS for ‘Avocet Susceptible’). B: Codominant STS markers amplified with primers Yr5STS-9 (5'-AAAGAATACTTTAATGAA-3') and Yr5STS-10 (5'-CAAACTTATCAGGATTAC-3'). The higher band (439 bp) was present in the resistant parent (the Yr5 NIL) and the resistant BC7:F3 bulk and the lower band (433 bp) was present in the susceptible BC7:F3 bulk and the susceptible parent (AVS).

View larger version (27K): [in a new window]   Fig. 3. Silver stained polyacrylamide gel showing association of the codominant markers amplified with STS primers Yr5STS-7 (5'-GTACAATTCACCTAGAGT-3') and Yr5STS-8 (5'-GCAAGTTTTCTCCCTATT-3') with the Yr5 locus. The higher band (478 bp) was present in the resistant parent (the Yr5 NIL), the homozygous resistant BC7:F3 lines, and the heterozygous BC7:F3 lines. The lower band (472 bp) was present in the susceptible parent (AVS), the homozygous susceptible BC7:F3 lines, and the heterozygous BC7:F3 lines. Phenotypic reactions of the parents and the progeny were evaluated with races PST-29 and PST-43 of Puccinia striiformis f. sp. tritici.

View larger version (65K): [in a new window]   Fig. 4. Ethidium bromide stained 1.5% (w/v) agrose gel showing undigested fragments of the Yr5 NIL and AVS and DpnII-digested fragments of the Yr5 NIL, AVS, and homozygous resistant (R), heterozygous (H), and homozygous susceptible (S) BC7:F3 lines amplified with the STS primers, Yr5STS-9 (5'-AAAGAATACTTTAATGAA-3') and Yr5STS-10 (5'-CAAACTTATCAGGATTAC-3'). M stands for 1-kp plus DNA size marker (Gibco BRL, Rockville, MD).