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Elevated Temperature and Carbon Dioxide Effects on Soybean Seed Composition and Transcript Abundance

^a Agronomy Dep., Univ. of Florida, Gainesville, FL 32611
^b USDA-ARS at the Agronomy Dep., Univ. of Florida, Gainesville, FL 32611
^c Agronomy Dep. and Plant Molecular and Cellular Biology Program, Univ. of Florida, Gainesville, FL 32611
^d School of Forest Resources and Conservation and Plant Molecular and Cellular Biology Program, Univ. of Florida, Gainesville, FL 32611

View larger version (31K): [in a new window]   Fig. 1. Soybean seed fresh weight vs. days after tagging in Exp. 1 at 350 or 700 µmol mol-1 [CO2]. The ellipse encircles the samples from 28/18 and 40/30°C, respectively, used for differential display (DD). In contrast, seed tested for composition had reached full maturity and had achieved maximum dry weight.

View larger version (27K): [in a new window]   Fig. 2. Mean oil concentration of mature soybean seed in Exp. 1 and Exp. 2 as affected by temperature at 350 or 700 µmol mol-1 [CO2].

View larger version (27K): [in a new window]   Fig. 3. Changes in oleic and linolenic fatty acid components of soybean seed oil as affected by temperature at 350 or 700 µmol mol-1 [CO2] in Exp. 1 (A) and Exp. 2 (B).

View larger version (22K): [in a new window]   Fig. 4. Concentration of nitrogen (A) and phosphorus (B) in soybean seed as affected by temperature at 350 or 700 µmol mol-1 [CO2].

View larger version (22K): [in a new window]   Fig. 5. Total nonstructural carbohydrate concentration (A), starch concentration (B), and soluble sugar concentration (C) of soybean seed as affected by temperature at 350 or 700 µmol mol-1 [CO2].

View larger version (30K): [in a new window]   Fig. 6. Steady state mRNA levels of ADR12 and ß-glucosidase gene homologs. (A) RNA gel blot data and (B) quantitation of Gm8 transcript abundance by reverse northern analysis.