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Mapping of the Ur-7 Gene for Specific Resistance to Rust in Common Bean

^a Dep. of Horticulture, Univ. of Nebraska, Lincoln, NE 68583
^b Dep. of Plant Pathology, Univ. of Nebraska, Lincoln, NE 68583
^c Life Sciences Informatics, Monsanto Company, St. Louis, MO 63167

View larger version (61K): [in a new window]   Fig. 1. Coupling-phase RAPD marker OAD12.550 expressing polymorphism between bulked DNAs from susceptible and resistant F2 plants, between the susceptible parent GN Nebr. #1 and the resistant parent GN1140, and between the susceptible line A 55 and the resistant line BelNeb-RR-1. 1 = GN Nebr. #1, 2 =GN1140, 3 = bulked DNA from susceptible F2 plants, 4 = bulked DNA from resistant F2 plants, 5 = A 55, 6 = BelNeb-RR-1, and 7 = molecular size marker.

View larger version (61K): [in a new window]   Fig. 2. Repulsion-phase RAPD marker OAB18.650 expressing polymorphism between bulked DNAs from susceptible and resistant F2 plants, between the susceptible parent GN Nebr. #1 and the resistant parent GN1140, and between the susceptible line A 55 and the resistant line BelNeb-RR-1. 1 = GN Nebr. #1, 2 =GN1140, 3 = bulked DNA from susceptible F2 plants, 4 = bulked DNA from resistant F2 plants, 5 = A 55, 6 = BelNeb-RR-1, and 7 = molecular size marker.

View larger version (15K): [in a new window]   Fig. 3. Linkage group 11 including the Ur-7 gene controlling specific rust resistance and six coupling-phase RAPD markers developed by means of an F2 population of the common bean cross GN1140 x Nebr. #1 (CGN), the Ur-7 gene and three repulsion-phase RAPD markers developed by means of the F2 population (RGN), and RAPD markers linked to the Ur-7 gene developed by means of a recombinant inbred line population of the cross BelNeb-RR-1 x A 55 (BA). The gene and marker names are given on the right and the length in centiMorgans is indicated on the left of linkage group 11. Markers OAD12.550, OAF17.900, and OAB18.650 in linkage group 11 are connected between the F2 and recombinant inbred line populations by lines.