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Electrolyte Leakage Assay Capable of Quantifying Freezing Resistance in Rose Clover

M. Eugénia S. Nunes*,a and G. Ray Smithb

a Faculdade de Ciências, Univ. do Porto, Rua do Monte Crasto Vairão, 4480 Vila do Conde, Portugal
b Texas A&M Univ. Agric. Res. and Ext. Ctr., P.O. Box 200, Overton, TX 77846, USA



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Fig. 1. Monthly precipitation, minimum and maximum temperatures at Overton, TX, 20-yr means.

 


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Fig. 2. Percentage electrolyte leakage measured in young leaves (YL) and older leaves (OL) of rose clover at Day 0 and 5 and 10 d after switching plants from COLD to WARM, from variable (VAR) to WARM, and from WARM to COLD, using three different test temperatures in Exp. 2. Error bars represent the SD where the error exceeds the size of the data symbol. Within each test temperature, mean values followed by the same letter are not significantly different at P < 0.01.

 


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Fig. 3. Percentage electrolyte leakage measured in young leaves (YL) and older leaves (OL) of rose clover at Day 0, and 2, 4, 7, 9 and 11 d after switching plants from WARM to COLD using five test temperature, in Exp. 3. Error bars represent the SD where the error exceeds the size of the data symbol.

 


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Fig. 4. Percentage electrolyte leakage measured in young leaves (YL) and older leaves (OL) of rose clover at Time 0, and 30, 60, and 90 min after being exposed to -12 and -14°C in Exp. 4. Error bars represent the SD where the error exceeds the size of the data symbol. Within each test temperature, mean values followed by the same letter are not significantly different at P < 0.01.

 


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Fig. 5. Percentage electrolyte leakage measured at seven sample dates (1 = 9 Feb., 2 = 22 Feb., 3 = 8 Mar., 4 = 18 Mar., 5 = 5 Apr., 6 = 19 Apr., and 7 = 4 May 1993) on leaves of rose clover growing in the field in Exp. 6. Results from three rose clover and one crimson cultivar are presented in (A) and results from five rose clover breeding lines are presented in (B).

 





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