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The PCR-Based Marker MWG2018 Linked to the RDG2A Leaf Stripe Resistance Gene Is a Useful Tool for Assessing Barley Resistance in Breeding Programs

Istituto Sperimentale per la Cerealicoltura, Sezione di Fiorenzuola, Via S. Protaso 302, I-29017 Fiorenzuola d'Arda (PC), Italy

View larger version (50K): [in a new window]   Fig. 1. Amplification products obtained by means of the STS primers MWG2018 and size-fractionated on ethidium bromide stained 5% (w/v) acrylamide gels. In (A) amplification products of the resistant genotypes: 1 Thibaut, 2 Rebelle, 3 Onice, 4 Proctor, 5 Vada, 6 Alf, 7 Rondo, 8 Diadem, 9 S3097, 10 Platine, 11 Murino, 12 Alpha, 13 Haruna Nijo, 14 Galleon. In (B) amplification products of the susceptible genotypes: 1 Mirco, 2 Jaidor, 3 CI6944, 4 Nudinka, 5 Passport. In (C) amplification products from five resistant BCC accessions. Size markers (50-bp DNA ladder, Gibco BRL) are shown at the left in the figures.

View larger version (54K): [in a new window]   Fig. 2. STS analysis of the marker verification lines derived from five crosses. A) Cross 1 (F3494 x Balkan): lines F6573, F6574 and F6575; Cross 2 (F3494 x Tamaris): lines F6576, F6577. B) Cross 3 (F3505 x Po609.35): lines 6571 and from 6578 to 6585. C) Cross 4 (Rebelle x Jaidor): lines F3485 and 3486; Cross 5 (Rebelle x F1341): lines F3510.A, F3510.B and F3510.C. R: resistant genotype; S: susceptible genotype. Genomic DNA was amplified with the STS primers MWG2018 and size-fractionated on ethidium bromide stained 5% acrylamide gels. Size markers (50-bp DNA ladder, Gibco BRL) are shown at the left in the figures.