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Genetic Mapping of the Or₅ Gene for Resistance to Orobanche Race E in Sunflower

^a Dep. of Crop and Soil Sci., Oregon State Univ., Corvallis, OR 97331-3002, USA
^b Pioneer Hi-Bred Int., Avda. Reino Unido s/n, Edificio Aditec 2a Planta, 41012 Sevilla, Spain
^c Pioneer Hi-Bred Int., Belediye Binasi Kat 3, Ahmetbey-Kirklareli, Turkey
^d Pioneer Hi-Bred Int., 18285 County Road 96, Woodland, CA 95695-9340, USA

View larger version (9K): [in a new window]   Fig. 1. Sunflower simple sequence repeat (SSR) marker genotypes amplified by polymerase chain recation (PCR) multiplex Sets 1 (Lanes 1–6), 11 (Lanes 7–12), and 13 (Lanes 13–18) on PHC, two Orobanche resistant PHC x PHD recombinant inbred line (RIL) bulks, two Orobanche susceptible PHC x PHD RIL bulks, and PHD (shown in order for each PCR-multiplex set). The white arrows highlight polymorphisms for three SSR markers on Linkage Group 3 (ORS1222 in Set 1, ORS1036 in Set 11, and ORS1114 in Set 13).

View larger version (9K): [in a new window]   Fig. 2. Genotypes for eight sunflower simple sequence repeat markers screened on PHC (Lane 1), an Orobanche resistant PHC x PHD recombinant inbred line (RIL) bulk (Lane 2), an Orobanche susceptible PHC x PHD RIL bulk (Lane 3), and PHD (Lane 4).

View larger version (41K): [in a new window]   Fig. 3. Composite (left) and PHC x PHD recombinant inbred line (right) maps for Linkage Group 3 of sunflower. Simple sequence repeat marker loci amplified by the polymerase chain reaction (PCR) multiplexes (ORS1036, ORS665, ORS1222, ORS949, and ORS1114) are shown in underlined boldface type.