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Physical and Genetic Mapping of Wheat Kinase Analogs and NBS-LRR Resistance Gene Analogs

^a Dep. of Plant Pathology, 4024 Throckmorton Hall, Kansas State University, Manhattan, KS 66506-5502
^b USDA-ARS Cereal Crops Research Unit, Northern Crop Science Laboratory, Fargo, ND 58105-5677
^c USDA-ARS Plant Science and Entomology Research Unit, Manhattan, KS 66506-5502

View larger version (65K): [in a new window]   Fig. 1. ClustalW alignments of nucleotide binding site-leucine-rich repeat (A) and kinase (B) containing resistance genes. Arrows identify the conserved motifs used to design primers. Positional identities of amino acids are highlighted in gray and dashes indicate computer generated gaps needed for alignments.

View larger version (103K): [in a new window]   Fig. 2. ClustalW alignments of amino acid (aa) sequences from the nucleotide binding site (NBS) RGAs cloned from Jagger and TA2460. These clones were compared with three NBS sequences from wheat Yr10, Cre3, and KSUD14, Xa1 from rice and Rp1-D from maize. Positional identities are highlighted in gray and computer generated gaps used for alignments are indicated by dashes.

View larger version (11K): [in a new window]   Fig. 3. An NBS protein tree derived from comparing predicted amino acid sequences of 8 NBS RGAs cloned from Jagger with three NBS genes from wheat, one from rice and one from maize. The tree was constructed using the nearest neighbor method. Percent occurrences during bootstrap analysis (from 1000 cycles) are indicated at each branch point.

View larger version (18K): [in a new window]   Fig. 4. A protein kinase tree constructed from comparing 26 kinase analogs cloned from Jagger and TA2460 with kinase resistance genes and other kinase sequences from rice, Arabidopsis, and wheat. Other kinases included, three wall associated kinases (wak) from Arabidopsis (Wak1, AJ009696; Wak2, AY062531; Wak4, AJ009695), Lrk10, Lrk19, Lrk33, and a putative (put) rice wak (AC113336). The tree was constructed using the nearest neighbor method and rooting was based on the midpoint. Percent occurrences during bootstrap analysis (from 1000 cycles) are indicated at each branch point.

View larger version (109K): [in a new window]   Fig. 5. Linkage maps of NBS RGAs and kinase RGLs. This map is based on the framework map of the ITMI population using LOD > 2.0. Markers are highlighted in bold. Markers that could not be placed with confidence were placed in the most likely interval.