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Pyramiding Quantitative Trait Locus (QTL) Alleles Determining Resistance to Barley Stripe Rust

Effects on Resistance at the Seedling Stage

^a Dep. of Crop and Soil Science, Oregon State University, 253 Crop Science Building, Corvallis, OR 97331-3002 and Departamento de Producción Vegetal, Est. Exp. "Dr. Mario A. Cassinoni", Facultad de Agronomía, Universidad de la República. Ruta 3, Km. 373, Paysandú 60000, Uruguay
^b Dep. of Crop and Soil Science, Oregon State University, 253 Crop Science Building, Corvallis, OR 97331-3002
^c USDA-ARS, Washington State University, Pullman, WA 99164-6430
^d Dep. of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717

View larger version (36K): [in a new window]   Fig. 1. Development of the barley stripe rust resistance QTL allele pyramid population. Black boxes represent resistance sources and white boxes represent susceptible parents. All of the mapping populations were composed of completely homozygous doubled-haploid lines.

View larger version (28K): [in a new window]   Fig. 2. Phenotypic distribution of infection type in the barley stripe rust resistance QTL pyramid population when inoculated at the seedling stage with four different isolates of Puccinia striiformis f. sp. hordei.

View larger version (24K): [in a new window]   Fig. 3. Alignment of the original mapping populations for the target regions on chromosomes 4(4H), 5(1H), and 7(5H), showing the markers used for genotyping the barley stripe rust resistance QTL pyramid population. "C/B" refers to Calicuchima/Bowman, "S/G" refers to Shyri/Galena. Markers indicated with larger font in the original maps were included to facilitate map alignment. Bmy1 and HvAmyB are different PCR-based markers derived from ß amylase-1 allele sequences.

View larger version (13K): [in a new window]   Fig. 4. Least squares means of the probability of occurrence of the susceptible phenotype in individuals with resistance alleles on QTL4, QTL5, and both QTL4 and QTL5, and with no resistance alleles. Data are shown for inoculation with each of four stripe rust isolates and for all isolates considered jointly.