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Genetic Mapping of Wheat Curl Mite Resistance Genes Cmc3 and Cmc4 in Common Wheat

^a Dep. of Entomology, Kansas State Univ., Manhattan, KS 66506, USA
^b USDA-ARS-NPA, Plant Science and Entomology Research Unit, Dep. of Agronomy, Kansas State Univ., Manhattan, KS 66506, USA
^c Dep. of Plant Pathology, Kansas State Univ., Manhattan, KS 66506, USA

View larger version (65K): [in a new window]   Fig. 1. Electrophoretic pattern of the dominant microsatellite marker SCM09 on WCM resistant wheat TAM 107, susceptible Tomahawk, and segregating F2 progenies. R and S indicate resistant and susceptible phenotype.

View larger version (14K): [in a new window]   Fig. 2. Genetic map of wheat chromosome 6DS. The dark region of the chromosome represents the Aegilops tauschii (TA2397) segment containing Cmc4 in common wheat germplasm KS96WGRC40. The light region represents the TAM 107 background.

View larger version (61K): [in a new window]   Fig. 3. Molecular markers closely linked to the Cmc4 locus in WGRC40. DNA polymorphisms as detected in WGRC40 (Cmc4), Wichita, TAM 107, TA2397, W304 (Cmc1), and Norstar. Arrows indicate size of the polymorphic fragments. (a) A codominant microsatellite GDM141 (b) a dominant RFLP detected by restriction endonuclease (XbaI) digestion of the genomic DNA and hybridized to the probe KSUG8 (c) polymorphism detected by microsatellite marker WMS904.

View larger version (68K): [in a new window]   Fig. 4. Polymorphism detected with a closely linked microsatellite marker GDM141 in hard winter wheat cultivars.