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Dehydrin Expression and Drought Tolerance in Seven Wheat Cultivars

^a Catedra de Mejoramiento Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de Lomas de Zamora, Ruta 4 Km. 2, Llavallol, 1832, Buenos Aires, Argentina
^b USDA/ARS, 3450 S.W. Campus Way, Corvallis, OR 97331
^c Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331-3002
^d Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331

View larger version (14K): [in a new window]   Fig. 1. Leaf water potential (MPa) in control and drought-treated wheat. Values are averages calculated from measurements of seven wheat cultivars in two independent experiments. Measurements were made on sampling dates 1 through 6 which represented seedlings at 16, 17, 18, 19, 20, and 25 d of age, respectively.

View larger version (82K): [in a new window]   Fig. 2. Expression of dehydrins in wheat leaves collected after 0 (A), 4 (B), 6 (C), and 12 (D) days of progressive drought stress in seedlings of cultivars Connie (C), Rhode (Rh), TAM105 (T), Hiller (H), Gene (G), Stephens (S), and Rod (R). Nonstress treatments consisted of well-watered plants treated identically to stressed plants in all other aspects. + = positive control (protein extract from Gene wheat subject to drought stress, used only on westerns A and D). Dehydrin proteins were detected using a commercial anti-dehydrin antibody prepared against the consensus dehydrin amino acid sequence EKKGIMDKIKELPG (Close et al., 1993).