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Molecular Mapping of Leaf Rust Resistance Gene Rph5 in Barley

J. A. Mammadova, J. C. Zwonitzerb, R. M. Biyasheva, C. A. Griffeya, Y. Jind, B. J. Steffensonc and M. A. Saghai Maroof*,a

a Crop & Soil Environmental Sciences Dep., Virginia Tech, Blacksburg, VA 24061
b The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401
c Dep. of Plant Pathology, Univ. of Minnesota, St. Paul, MN 55108-6030
d Plant Science Dep., South Dakota State Univ., Brookings, SD 57007



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Fig. 1. Partial molecular maps of barley chromosome 3H showing the genetic location of leaf rust resistance gene Rph5. Markers were mapped in two segregating populations: (A) Bowman (rph5) x Magnif 102 (Rph5); (B) Moore (rph5) x Virginia 92-42-46 (Rph5), respectively. Map distances are given in centimorgans (cM).

 


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Fig. 2. RFLP analysis of Bowman (rph5), the NIL Magnif 102/8*Bowman (Rph5), Magnif 102 (Rph5) and Quinn (Rph5 and Rph2) with VT1.

 


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Fig. 3. Estimation of the relative locations of Rph5 and Rph7 leaf rust resistance genes on the basis of comparison of three maps with common markers. Maps of Moore x Virginia 92-42-46 and Bowman x Magnif 102 are from this study, and Cebada Capa x Bowman map was published recently (Brunner et al., 2000).

 





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