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Development of Species-Specific SCAR Markers in Bentgrass

^a Dep. of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706
^b Dep. of Agronomy, University of Wisconsin-Madison, Madison, WI 53706

View larger version (132K): [in a new window]   Fig. 1. RAPD banding patterns of Operon primers AF6(a) and P8(b) and 14 bentgrass DNAs. Arrows indicate species-specific, strong and reproducible RAPD bands that were amplified in creeping(a) and colonial(b) bentgrass species and converted into species-specific SCAR markers. Bentgrass plants indicated are two velvet bentgrass plants of cultivar EVM 99 (Lanes 1 and 2), seven creeping bentgrass plants of Cato (Lanes 3–9), and five colonial bentgrass plants of ‘9F7’ (Lanes 10–14). "M" indicates 100-bp marker with a bright middle band estimating 600 bp.

View larger version (118K): [in a new window]   Fig. 2. Banding pattern of SCAR primer pairs Creep700F/R(a) and Col400F/R(b) amplifying a single and reproducible band specifically for creeping and colonial bentgrass species, respectively. Optimal annealing temperature is at 65°C for both sets of SCAR primers. Bentgrass plants indicated are three velvet plants of cultivar EVM 99 (Lanes 1 and 2), 7 creeping plants of Cato (Lanes 3–9), and five colonial plants of 9F7 (Lanes 10–14). M indicates 100-bp marker with bright middle band indicating 600 bp.