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Genetic Analysis and Random Amplified Polymorphic DNA Markers Associated with Cooking Time in Common Bean

Carmen Jacinto-Hernandeza, Susana Azpiroz-Riveroa, Jorge A. Acosta-Gallegosa, Humberto Hernandez-Sanchezb and Irma Bernal-Lugo*,c

a CEVAMEX, Apdo. 10-56230 Chapingo México
b Departamento de Graduados e Investigacion en Alimentos, ENCB, IPN
c Facultad de Química, UNAM, Ciudad universitaria, D.F. 04510, México



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Fig. 1. Frequency distribution of the RILs from BM xBV for cooking time. Arrows indicate the cooking time for the parents.

 


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Fig. 2. RAPD amplification of a RILs population of BM x BV. Parents BM and BV, lanes 1-2 respectively; lanes 3-21, different RILs. Key to individuals: RILS showing short cooking time, 4 to 8, 10, 12 to 15, 16 to 17, 19 (A), 1 to 2, 4 to 6, 8 to 11, 14 to 17 (B), 1 to 7, 10 to 17, 19 (C). The others are RILS showing long cooking time. The arrow indicates the RAPD band with molecular weight of approximately 310 bp (UNAM-16) which is only amplified in the short cooking parent. Lanes m1 through m2 molcular size markers ({phi} X174 and {lambda} HindIII, respectively; size of band indicated in bp).

 





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