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Alfalfa Root Nitrogen Reserves and Regrowth Potential in Response to Fall Harvests

^a Département de Phytologie, Université Laval, Sainte-Foy, QC, Canada, G1K 7P4
^b Agriculture et Agroalimentaire Canada, Sainte-Foy, QC, Canada, G1V 2J3

View larger version (66K): [in a new window]   Fig. 1. Root concentrations of total N (A, B), total amino acids (C, D), and total soluble proteins (E, F) of two alfalfa cultivars (AC Caribou and WL 225) harvested twice or three times with a third harvest taken at 400, 500, or 600 GDD after the second harvest. Concentrations were measured at each harvest date for the corresponding harvest treatments (Harvests): on 7 Aug. 1997 for plants harvested twice, and on 8 Sept., 17 Sept., or 7 Oct. 1997 for plants harvested at 400, 500, or 600 GDD after the second harvest, respectively. Concentrations were also measured for all harvest treatments on 10 Nov. 1997, 12 Jan. 1998, and 11 Mar. 1998. Vertical bars indicate SEM for each sampling date, except for soluble proteins since there was no replicate.

View larger version (51K): [in a new window]   Fig. 2. Root concentrations of asparagine (A, B), proline (C, D), arginine (E, F), and histidine (G, H) of two alfalfa cultivars (AC Caribou and WL 225) harvested twice or three times with the third harvest taken at 400, 500, or 600 GDD after the second harvest. Concentrations were measured at each harvest date for the corresponding harvest treatments (Harvests): on 7 Aug. 1997 for plants harvested twice, and on 8 Sept., 17 Sept., or 7 Oct. 1997 for plants harvested at 400, 500, or 600 GDD after the second harvest, respectively. Concentrations were also measured for all harvest treatments on 10 Nov. 1997, 12 Jan. 1998, and 11 Mar. 1998. Vertical bars indicate SEM for each sampling date.

View larger version (54K): [in a new window]   Fig. 3. Root pools of total N (A, B), total amino acids (C, D) and total soluble proteins (E, F) of two alfalfa cultivars (AC Caribou and WL 225) harvested twice or three times with the third harvest taken at 400, 500, or 600 GDD after the second harvest. Pools were calculated at each harvest date for the corresponding harvest treatments (Harvests): on 7 Aug. 1997 for plants harvested twice, and on 8 Sept., 17 Sept., and 7 Oct. 1997 for plants harvested at 400, 500, or 600 GDD after the second harvest, respectively. Pools were also calculated for all harvest treatments on 10 Nov. 1997, 12 Jan. 1998, and 11 Mar. 1998. Vertical bars indicate SEM for each sampling date, except for soluble proteins since there was no replicate.

View larger version (40K): [in a new window]   Fig. 4. Root pools of asparagine (A, B), proline (C, D), arginine (E, F), and histidine (G, H) of two alfalfa cultivars (AC Caribou and WL 225) harvested twice or three times with the third harvest taken at 400, 500, or 600 GDD after the second harvest. Pools were calculated at each harvest date for the corresponding harvest treatments (Harvests): on 7 Aug. 1997 for plants harvested twice, and on 8 Sept., 17 Sept., and 7 Oct. 1997 for plants harvested at 400, 500, or 600 GDD after the second harvest, respectively. Pools were also calculated for all harvest treatments on 10 Nov. 1997, 12 Jan. 1998, and 11 Mar. 1998. Vertical bars indicate SEM for each sampling date.

View larger version (78K): [in a new window]   Fig. 5. Seasonal changes in SDS-PAGE profiles of soluble proteins in roots of alfalfa cultivar AC Caribou harvested twice in the summer (A), or harvested a third time after the second harvest at 400 GDD (B), 500 GDD (C), or 600 GDD (D). Lane 1: 2nd harvest, 7 Aug. 1997; Lane 2: 3rd harvests, 8 Sept. 1997 (B), 17 Sept. 1997 (C), and 7 Oct. 1997 (D); Lane 3: 10 Nov. 1997; Lane 4: 12 Jan. 1998; Lanes 5: 11 Mar. 1998. Molecular weight standards listed to the left are 97.4, 66.2, 45, 31, 21.5, and 14.4 kDa.