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Genotypic Variation for Glycinebetaine in Sorghum

Wen-Ju Yanga, Patrick J. Richa, John D. Axtellb, Karl V. Woodc, Connie C. Bonhamd, Gebisa Ejeta, Michael V. Mickelbart*,e and David Rhodesa

a Dep. of Horticulture and Landscape Architecture, Purdue Univ., West Lafayette, IN 47907
b Dep. of Agronomy, Purdue Univ., West Lafayette, IN 47907
c Dep. of Chemistry, Purdue Univ., West Lafayette, IN 47907
d Dep. of Biochemistry, Purdue Univ., West Lafayette, IN 47907
e Soil, Plant, and Ecological Sciences Division, Lincoln University, Canterbury, New Zealand



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  		Fig. 1. Frequency distribution of total quaternary ammonium compound (QAC) levels (determined by the periodide assay) from the flag leaves of 240 sorghum genotypes in the 1989 growing season. Individual QAC levels for all 240 genotypes tested are presented in Table 1.

 


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  		Fig. 2. Total quaternary ammonium compound (QAC) levels ( ± SE mean) in betaine fraction of young expanding leaves of sorghum genotypes IS2319 (open bars) and P932296 (solid bars). Plants were grown for a total of 21, 28, 35, 42, or 49 d (bottom row, x axis) and were salinized with 100 mM NaCl for 0, 7, or 14 d (top row, x axis) before leaves were harvested for QAC isolation and quantification. Total QAC levels were determined in three individual plants by the colorimetric periodide assay after purification of the betaine fraction from plants.

 




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