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Use of digital image analysis, viability stains, and germination assays to estimate conventional and glyphosate-resistant cotton pollen viability

Botany Dep., North Carolina State Univ., Raleigh, NC 27695

View larger version (92K): [in a new window]   Fig. 1. Comparison of pollen grains using different viability assays. A. Viable, germinated pollen grain assessed by the B & K method and phase contrast microscopy. B. Viable and nonviable pollen (partially stained, arrows) assessed by Alexander's stain method. C. Viable, fluorescing pollen assessed by FCR method. D. Irregularly stained, nonviable pollen grain from glyphosate-treated DP 5415RR plant assessed by FCR method. Scale bars are 50 µm.

View larger version (25K): [in a new window]   Fig. 2. Regression of percent pollen viability to seeds per boll for four different pollen viability assays. Three-parameter logistic curves were fit to data. Each data point represents the pollen viability and corresponding seed set per boll for one of the four methods. Equations of curves and R2 are as follows: B & K method, y = 155.9/(1 + (x/27.2)-1.29), R2 = 0.57; Alexander stain, y = 150.9/[1 + (x/15.3)-0.74], R2 = 0.61; B & K + aniline blue method, y = 237.8/[1 + (x/68)-0.95], R2 = 0.47; and FCR method, y = 103.3/[1 + (x/20.8)-4.3], R2 = 0.63.