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Development of Dominant Rice Blast Pi-ta Resistance Gene Markers

^a USDA-ARS, Dale Bumpers National Rice Research Center, P. O. Box 1090, Stuttgart, AR 72160-0287
^b Institute of Nuclear Agricultural Sciences, Zhejiang Univ., Hangzhou, P. R. China 310029

View larger version (59K): [in a new window]   Fig. 1. A schematic representation of the double strands of the 6927 bp of the Pi-ta gene (A) and PCR products from genomic DNA prepared from Katy (1), Drew (2), Kaybonnet (3), Nipponbare (4), and M-202 (5) amplified by the dominant Pi-ta-specific primers. Line 6 is the water control (B). Names and locations of primers and region amplified by PCR using primers YL153 and YL154 (i), YL155/YL87 (ii), and YL100/YL102 (iii) are shown in A. The sizes of fragments were estimated by mean of kilobase markers. ATG indicates the translation start site and TGA indicates the termination site.