HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Rahman, M. Articles by Zafar, Y. Search for Related Content PubMed Articles by Rahman, M. Articles by Zafar, Y. Agricola Articles by Rahman, M. Articles by Zafar, Y. Related Collections Plant Disease Plant Genetic Resources Crop Genetics

Estimation of Genetic Divergence among Elite Cotton Cultivars–Genotypes by DNA Fingerprinting Technology

^a National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad, Pakistan
^b Dep. of Genetics, Univ. of Melbourne Parkvill,Victoria 3052, Australia

View larger version (123K): [in a new window]   Fig. 1. Amplification of two geminivirus species Cotton leaf curl virus—Pakistan 1 (CLCuV-Pk1) and Cotton leaf curl virus—Pakistan2 (CLCuV-Pk2) by multiplex PCR. The upper band is the PCR product from CLCuV-Pk2 (510 bp) with primers CLCuV-V2091 and PCL2, and the lower band (360 bp) is the PCR product from CLCuV-Pk1 with primers CLCuV-V2091 and CLCuVPk1-C2442. A. Lane M = kb Ladder, Lane 1 = FH-901, Lane 2 = CIM-473, Lane 3 = CIM-482, Lane 4 = CIM-240, Lane 5 = NIAB-98, Lane 6 = MNH-554, Lane 7 = FH-945, Lane 8 = CIM-448, Lane 9 = RH-500, Lane 10 = VH-53, Lane 11 = FH-930, Lane 12 = BH-36, Lane 13 = positive control (radish DNA), Lane M = kb ladder. B. Lane M = kb ladder, Lane 1 = NF-801, Lane 2 = CIM-435, Lane 3 = NIAB-Karishma, Lane 4 = MNH-93, Lane 5 = S-12, Lane 6 = FH-900, Lane 7 = MNH-552, Lane 8 = CIM-443, Lane 9 = BH-118, Lane 10 = CIM-1100, Lane 11 = VH-137, Lane 12 = CP-15/2, Lane 13 = Cedix, Lane 14 = LRA-5166, Lane 15 = positive control (radish DNA), Lane 16 = negative control, M = kb ladder.

View larger version (100K): [in a new window]   Fig. 2. Dot-blot hybridization signals of the cotton genotypes. In each row one well has been empty after the first one. 1A = positive control (radish DNA), 3A = negative control, 5A = BH-36, 7A = FH-900, 9A = MNH-552, 2B = CIM-443, 4B = BH-118, 6B = VH-137, 8B = FH-901, 10B = CIM-473, 1C = NIAB-98, 3C = CIM-482, 5C = FH-930, 7C = FH-945, 9C = NF-801, 2D = CIM-1100, 4D = S-12, 6D = CIM-448, 8D = RH-500, 10D = CP-15/2, 1E = CIM-435, 3E = Cedix, 5E = LRA-5166, 7E = VH-53, 9E = Ravi, 2F = CIM-240, 4F = MNH-93, 6F = MNH-554, 8F = NIAB-Karishma, 10F = Empty.

View larger version (94K): [in a new window]   Fig. 3. Amplification profile of 20 cotton genotypes with primer OPA-10. M = kb ladder, 1 = FH-900, 2 = MNH-552, 3 = CIM-443, 4 = BH-118, 5 = VH-137, 6 = FH-901, 7 = CIM-473, 8 = CIM-482, 9 = NF-801, 10 = FH-930, 11 = FH-945, 12 = MNH-554, 13 = CIM-1100, 14 = CIM-448, 15 = NIAB-98, 16 = RH-500, 17 = CP 15/2, 18 = Cedix, 19 = LRA-5166, 20 = CIM-435, M = kb ladder.

View larger version (84K): [in a new window]   Fig. 4. M = kb ladder, 1 = FH-900, 2 = MNH-552, 3 = CIM-443, 4 = BH-118, 5 = VH-137, 6 = FH-901, 7 = CIM-473, 8 = CIM-482, 9 = NF-801, 10 = FH-930, 11 = FH-945, 12 = MNH-554, 13 = CIM-1100, 14 = CIM-448, 15 = NIAB-98, 16 = RH-500, 17 = cp 15/2, 18 = cedix, 19 = LRA-5166, 20 = CIM-435, m = kb ladder.

View larger version (24K): [in a new window]   Fig. 5. Dendrogram of 20 cotton genotypes developed from RAPD data using the unweighted pair group method of arithmetic means (UPGMA). The scale is based on Nei and Li's coefficients of similarity.