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Mapping and Progress toward Map-Based Cloning of Brown Planthopper Biotype-4 Resistance Gene Introgressed from Oryza officinalis into Cultivated Rice, O. sativa

^a Dep. of Soil and Crop Sciences, Texas A&M Univ., College Station, TX 77843
^b Dep. of Agronomy, Kansas State Univ., Manhattan, KS 66506
^c Dow Agro Sciences, 9330 Zionsville Road, Indianapolis, IN 46268-1054
^d Dep. of Plant Breeding and Biometry, Cornell Univ., Ithaca, NY 14853-1901
^e Centre for Plant Molecular Biology, Tamil Nadu Agricultural Univ., Coimbatore 641 003, India

View larger version (115K): [in a new window]   Fig. 1. Polymorphic RAPD pattern obtained from random 10mers. R-resistant parent; S-susceptible parent; 1-10 resistant progenies and 11-20 susceptible progenies. Random primers AJ09, AL05, AL01 and AK10 generated 4 (a260, b230, c180 & d100), 2 (a220 & b400), 1 (a200) and 4 (a690, b430, c380, and d340) polymorphic markers, respectively, for BPH resistance. The polymorphic markers are indicated by arrows.

View larger version (17K): [in a new window]   Fig. 2. Linkage map of Bph13 (t) locus. Left is the standard IR64 x Azucena map (Temnykh et al., 2000) and right is the map constructed for Bph13 (t) locus.

View larger version (35K): [in a new window]   Fig. 3. Conversion of AJ09b into PCR-based STS marker and its cosegregation with BPH resistance. R-resistant parent; S-susceptible parent; 1-20 resistant progenies and 21 to 38 susceptible progenies. PCR amplification of STS primer generated 200-bp fragment in resistant parent and resistant individuals and 179-bp fragment in susceptible parent and susceptible individuals.