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Modified Rapid DNA Extraction Protocol for High Throughput Microsatellite Analysis in Wheat

USDA-ARS Plant Science and Entomology Unit, 4008 Throckmorton Hall, Manhattan, KS 66506

View larger version (113K): [in a new window]   Fig. 1. Four-day-old germinating wheat seedlings in an 8-well culture plate.

View larger version (68K): [in a new window]   Fig. 2. A) Electropherograms generated from ABI 3100 Prism genetic analyzer. 1—Marker gwm493-W-7984 control CTAB extraction. 2—Marker gwm493 - Opata control CTAB extraction. B) 2.3% SFR gel electrophoresis of rapid DNA extraction amplification with gwm493. 1—W-7984, 1 min. maceration; 2—W-7984, 3 min. maceration; 3—W-7984, 5 min. maceration; 4—W-7984, 7 min. maceration; 5—W-7984, 10 min maceration; 6—W-7984, CTAB control; 7—Opata, 1 min. maceration; 8—Opata, 3 min. maceration; 9—Opata, 5 min. maceration; 10—Opata, 7 min. maceration; 11—Opata, 10 min. maceration; 12—Opata, CTAB control.