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Alignment of Lipid Bodies along the Plasma Membrane during the Acquisition of Desiccation Tolerance in Maize Seed

^a Colegio de Postgraduados (IREGEP), Montecillo Edo. de Mexico, 56230, Mexico
^b Burris Consulting, 1707 Burnett Ave., Ames, IA 50010

View larger version (100K): [in a new window]   Fig. 1. Illustration of tissues and lipid bodies in radicle meristem of maize embryo axes: (A) longitudinal section of radicle meristem (Bar = 80 µm, arrow indicates the sectioning location); (B) cross section of radicle meristem (Bar = 80 µm), numbers indicate locations from where micrographs were taken [root cap cells (1), outer (2), and inner (3) QC cells]; (C) Scanning electron micrograph where lipid bodies can be observed in three-dimension; (D) Contrasting visualization of one-dimension view of lipid bodies in TEM, sectioning in left cell occurred far from cell surface and sectioning in right cell occurred just below cell surface. Bar = 2 µm. Arrows point out lipid bodies. A and C photographs were courtesy of Dr. J.A. Hartwigsen.

View larger version (312K): [in a new window]   Fig. 2. Movement of lipid bodies toward the cell wall during preconditioning and field maturation in cells of the embryo radicle: root cap cells (A), outer (B) and inner (C) QC cells of seed harvested in 2000 at 500 g H2O kg-1 fw without PC; after 24 h PC root cap cells (D), outer (E) and inner (F) QC cells; after 48 h outer (G) and inner (H) QC cells; After 72 h PC inner QC cells (I); outer QC cells (J) of seed harvested at 400 g H2O kg-1 fw; outer (K) and inner (L) QC cells of seed harvested at 320 g H2O kg-1 fw. Bar = 2 µm and arrows point at lipid bodies.

View larger version (28K): [in a new window]   Fig. 3. Moisture loss in intact seed and embryo under different drying conditions, (A) seed harvested at 500 g H2O kg-1 fw and dried at the preconditioning (PC) drying conditions, preconditioning phase denote those treatments that were transfer to fluidized bed (FB) drying; (B and C) seed harvested at 500 and 320 g H2O kg-1 fw, respectively, both subjected to FB drying without PC. Bars denote standard error of the mean.

View larger version (154K): [in a new window]   Fig. 4. Alignment of lipid bodies along the plasma membrane in inner QC cells of the embryo radicle after the seed was dried under different conditions in 1998, (A, B and C) seed harvested at 550, 400, and 320 g H2O kg-1 fw, respectively, and dried in the fluidized bed without PC; (D and E) seed harvested at 550 and 500 g H2O kg-1 fw, respectively both with 48 h PC; (F) seed harvested at 500 g H2O kg-1 fw dried entirely at PC (35C) conditions. Bar = 2 µm and arrows point at lipid bodies.

View larger version (32K): [in a new window]   Fig. 5. Electrical conductivity (A), standard germination test (B) and shoot to root ratio (C) of seed harvested at 550, 500, 400, and 320 g H2O kg-1 fw) and subjected to preconditioning (PC) drying times before fast drying. 35C = drying the ears the entire period at the PC conditions, NH = drying the ears the entire period with unheated air. Bars denote standard error of the mean.

View larger version (22K): [in a new window]   Fig. 6. Accumulation of dry matter in seed (SDW) and embryo (EDW) of seed harvested at different moisture contents. Bars denote standard error of the mean.